In this study, fixed oil is extracted from the seeds of Putranjiva roxburghii. Wall and has been evaluated the percentage yield, physical and chemical properties of the extracted oil. The highest percentage of oil obtained was 50% w/v identified after different extraction. The physicochemical property of oil suggests the use of this oil in liquid soap, shampoo and oil based ice cream production. The antimicrobial activity of the oil was evaluated using disc diffusion method. Results showed that the oil exhibited significant antibacterial activity against Staph.aureus and antifungal activity against C. albicans.
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